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KMID : 0811720180220020163
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Korean Journal of Physiology & Pharmacology
2018 Volume.22 No. 2 p.163 ~ p.172
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Effects of salmon DNA fraction in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model
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Ra Ho-Jong
Oh Mi-Young
Kim Hee-Ju
Lee Seung-Yong
Eom Dae-Woon
Lee Suk-Kyu
Kim Su-Nam
Chung Kyu-Sung
Jang Hyuk-Jai
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Abstract
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PRF001 is a fragmented DNA polymer extracted from the testes of salmon. The purpose of this study was to assess the anti-inflammatory effect of PRF001 in vitro as well as the protective effect of PRF001 intake against arthritis in a rat model. In vitro, cell survival and inflammatory markers after H2O2 treatment to induce cell damage were investigated in CHON-001 cells treated with different concentrations of PRF001. In vivo, osteoarthritis was induced by intra-articular injection of monosodium iodoacetate (MIA) into the knee joints of rats. After consumption of PRF001 (10, 50, or 100 mg/kg) for 4 weeks, inflammatory mediators and cytokines in articular cartilage were investigated. In vitro, the levels of inflammatory markers, IL-1β, TNF-α, COX-2, iNOS, and PGE2, were significantly suppressed by PRF001 treatment. In vivo, the inflammatory mediators and cytokines, IL-1β, p- Erk1/2, NF-κB, TNF-α, COX-2, and PGE2, as well as MMP3 and MMP7, which have catabolic activity in chondrocytes, were decreased in the MIA-induced osteoarthritic rats following intake of PRF001. Histological analysis revealed that PRF001 had a protective effect on the articular cartilage. Altogether, these results demonstrated that the anti-inflammatory property of PRF001 contributes to its protective effects in osteoarthritis through deregulating IL-1β, TNF-α, and subsequent signals, such as p-Erk1/2, NF-κB, COX-2, PGE2, and MMPs.
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KEYWORD
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Chondrocyte, Cytokine, Inflammation, Osteoarthritis, Polydeoxyribonucleotide, PRF001
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